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KMID : 0380620220540050546
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Korean Journal of Food Science and Technology
2022 Volume.54 No. 5 p.546 ~ p.550
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Preparation of selective medium for yeast and lactic acid bacteria by adding antibacterial and antifungal agents
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Park Ho-Min
Cha Jeong-Min
Bae Soh-Won
Son Hong-Seok
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Abstract
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In this study, we examined whether the of yeast mold agar and de Man, Rogosa and Sharpe (MRS) agar was improved when antibacterial and antifungal agents were added. The addition of 0.01 g/L of chloramphenicol, a widely used antibacterial agent, was sufficient to inhibit the growth of lactic acid bacteria (LAB), and the addition 0.5 g/L of the antifungal agent, cycloheximide, inhibited yeast growth. The antifungal agents, amphotericin B and methyl paraben, poorly inhibited yeast growth at concentrations of 0.5 and 0.28 g/L, respectively. Moreover, it was shown that the addition of chloramphenicol or cycloheximide selectively inhibited the growth of LAB or yeast in the makgeolli sample, whereby both yeast and LAB were present. These results suggest that these selective media can be used to count in various types of samples in which LAB and yeast coexist.
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KEYWORD
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selective medium, chloramphenicol, cycloheximide, yeast mold, MRS
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